Nephroprotective Effects of Ethanolic Extracts of Azadirachta Indica Seeds and Leaves in Diabetic Rats

Abstract

Objective: To evaluate and compare the outcomes of effects of Azadirachta indica on serum urea and
creatinine in alloxan induced diabetic albino rats.
Method: The study was a randomized controlled trail carried out in Physiology department of services
institute of medi-cal sciences, Lahore from November 2018 to April 2019. 120 male albino rats were
randomly and uniformly divided in four groups (n=30). Diabetic control and experimental groups were
administered with alloxan monohydrate intraperitoneal injection of (120mg/kg) to induce diabetes mellitus.
G1 (control) received normal saline orally; G2 was (diabetic control), group 3 received Neem leaves extract
(500 mg/kg body weight); and group 4 received Neem seeds extract (500 mg/kg body weight) as a single dose
for four weeks. Subsequently blood samples (4-5ml intracardiac) were collected from each group member on
29th day to evaluate the biochemical parameters of serum urea and creatinine.
Results: The ethanol based extracts of Neem seeds and leaves lead to highly significant (p˂0.001) reduction
in urea and creatinine levels of G3 and G4.
Conclusion: Azadirachta indica leaves and seeds can significantly contribute in lowering serum urea and
creatinine

How to cite: Saleem T, Saleem A, Jabeen N, Saleem N, Khalid N, Ikram O. Nephroprotective Effects of Ethanolic Extracts
of Azadirachta Indica Seeds and Leaves in Diabetic Rats. Esculapio - JSIMS 2022;18(02):179-183
DOI: https://doi.org/10.51273/esc22.2518215