Effects of Flax Seed Oil on Histological & Biochemical Metamorphosis Induced by Caffeinated Energy Drink in Adult male Albino Rats Bone

Abstract

Objective: To appraise protective effects of flax seed oil on caffeinated energy drink induced histological
(osteoporotic cavities) & biochemical (Alkaline phosphatase) changes in adult male albino rats bone
Method: This was an experimental study conducted at department of Anatomy Shaikh Zayed Postgraduate
Medical Institute Lahore for 8 weeks. 32 adult male albino rats weighing (250-300gm) were randomly
divided into four groups. Group A (Control) received corn oil 5ml/kg body weight in addition to basal diet
daily for 8 weeks. Group B (Experimental) received caffeinated energy drink (15ml/kg body weight) and
corn oil (5ml/kg body weight) for 8 weeks. Group C (Experimental) received caffeinated energy drink (15
ml/kg body weight) and 40% flax seed oil (5ml/kg body weight), while group D (Experimental) received
caffeinated energy drink (15ml/kg body weight) and 60% flax seed oil (5ml/kg body weight) daily for 8
weeks respectively. All animals were weighed before and after experiment. Blood samples were taken before
and after 24 hours of giving last doses for estimation of serum ALP. Furthermore, right femora were used for
histological purpose.
Results: Significant difference in mean osteoporotic cavities of femora among experimental groups (p =
0.000) was observed. Insignificant difference was found in mean serum ALP (p = 0.072).
Conclusion: Flax seed oil helped in alleviating osteoporotic changes induced by caffeinated energy drink in
femora of adult male albino rats.

How to cite: Waseem A, Suhail M, Batool A, Zaheer A, Rehman A, Bilal A. Effects of Flax Seed Oil on Histological & Biochemical
Metamorphosis Induced by Caffeinated Energy Drink in Adult male Albino Rats Bone. Esculapio - JSIMS 2022;18(02):152-157
DOI:https://doi.org/10.51273/esc22.2518210