Effect Of Storage Conditions On The Morphological Preservation Of Rabbit Kidney And Liver Tissues

Abstract

Objective: To see the effect of different solutions and storage temperature on morphological
preservation of rabbit kidney and liver tissues.
Material and Methods: In this experimental study, rabbit kidney and liver slices were stored in
Ringer Lactate, Euro Collins and University of Wisconsin solutions (UW solution), for various
fixed time intervals. The three solutions were tried at room temperature as well as at 0-4°C.
Morphological preservation was assessed by a semi quantitative method.
Results: Adequate morphological preservation of both tissues was obtained in all solutions at
room temperature for the first 24 hours. Lowering of storage temperature to 0-4°C was highly
beneficial to both tissues in all solutions. UW solution is more effective for liver tissue
preservation.
Conclusion: Anyone of the solutions may be used at room temperature, for preservation of
tissue to be submitted for immuno-histochemistry for the first 24 hours. If prolonged storage is
required, lowering of temperature is recommended.