Effects of Avocado Aqueous Seed Extract on Liver Biochemical Markers in Rats with Hepatotoxicity Induced by Isoniazid

Abstract

Objective: To observe the improvement in serum biomarkers of liver function (LFTs) by using aqueous
Avocado seed extract with isoniazid (INH) in Albino rats.
Method: It was an experimental research conducted at KEMU from January to June 2019. The study
included thirty six male Sprague Dawley Albino rats divided into 4 group using lottery method, each group
having 9 animals. All animal groups were administered treatment by gavage method for 30 days. Blood
sample of each animal for biochemical markers analysis was collected 24 hours after the last dose of drugs by
cardiac puncture. The collected data from the four groups was entered in Statistical package for social
sciences (SPSS) version 26 for analysis. Mean and standard deviation were calculated for quantitative
characteristics. One way ANOVA was used to make the comparison between all groups. Pair wise comparison
was performed using Least square difference (LSD) Test. P-value <0.05 was considered significant.
Results: Serum alanine aminotransferase (ALT), serum aspartate aminotransferase (AST), serum alkaline
phosphatase (ALP), and serum bilirubin showed a significant difference upon co-administration of aqueous
Avocado seed extract with INH among all the groups.
Conclusion: The current study indicates a significant dose-dependent improvement in serum biomarkers of
liver function when INH and avocado aqueous seed extract are used together.

How to cite: Bashir Z, Abbasi SQ, Aslam A, Kanwal S, Tahweez R, Mansoor G. Effects of Avocado Aqueous Seed Extract on Liver
Biochemical Markers in Rats with Hepatotoxicity Induced by Isoniazid. Esculapio - JSIMS 2022;18(03):355-359