Protective Effects of Moringa Oleifera Leaves Extract on Bisphenol A Induced Changes in the Proximal Convoluted Tubules of The Kidneys of Adult Albino Rats

Abstract

Objective: To determine the effects of Moringa oleifera leaves extract on Bisphenol A induced
changes in the PCT diameters and PCT cell vacuolization in the kidneys of adult albino rats.
Methods: Thirty adult albino Wistar rats of any gender, weighing (160-180g) were acquired from
National Institute of Health, Islamabad. 30 rats were randomly divided into 3 equal groups as A, B
and C, so that each group had 10 rats. Group A rats received standard rat feed and 1ml/kg of
distilled water via oral gavage only. Group B received BPA at dose of 50mg/kg/day. Group C
received BPA at dose of 50mg/kg/day, followed by MoLE at dose of 500mg/kg/day. All animals
were sacrificed at the end of 8 weeks. The kidneys were dissected out and processed further for
H&E staining and oculo micrometry was done to measure the PCT diameter and observe the PCT
cell vacuolizations.
Results: The increase in diameters of PCT and PCT cell vacuolization, that was seen in group B
upon giving BPA only was prevented in the animals of group C that was given MoLE with BPA.
Conclusions: Moringa oleifera leaves extract prevents BPA induced increase in the PCT
diameters and PCT cells vacuolizations in the kidneys of adult albino rats