Correlation of Serum Adiponectin with Lipid profile in Diabetic and Non Diabetic Rats

Abstract

Objective: To determine the relationship of adiponectin with lipid profile in diabetic and non
diabetic rats.
Methods: A randomized controlled trial (RCT) study was conducted at Physiology department,
Services Institute of Medical Sciences (SIMS), Lahore from August 2013 to January, 2014. This
study was conducted on sixty male albino rats weighing 150-200gm.
Thirty rats were selected after induction of diabetes with alloxan monohydrate (group B). Another
30 non diabetic healthy rats were chosen and labeled as group A. A 4-5 ml intracardiac blood
sample was taken from each rat. Biochemical parameters evaluated were adiponectin (by ELISA
method), serum total cholesterol (by CHOD-PAP enzymatic colorimetric method), serum
triglyceride (by GPO-PAP enzymatic colorimetric method), serum HDL-C (by Precipitation
method) and serum LDL C (by using Friedewald fomula, LDL=TC- (HDL+TG/5).
Results: There was a significant negative (r= -.404, p=.027) correlation of serum adiponectin
with serum triglyceride in diabetic group.
Serum VLDL also showed a significant negative (r= -.404, p=.027) correlation with serum
adiponectin in diabetic group. No significant correlation was found between serum adiponectin
and lipid parameters in non diabetic group.
Conclusions: The negative correlation of serum adiponection with serum triglycerides and
VLDL in diabetics depicts its association with insulin resistance in type 2 diabetics.