Prophylactic Anti-Ulcer Effect of Punica Granatum (Pomegranate) Peel and Seed Extract in Murine Peptic UlcerModel

Abstract

Objective: To assessthe prophylactic antiulcer effect ofmethanolic extracts of Punica granatum(Pomegranate) peel and seed and its comparison to pantoprazole in diclofenac induced murine model of peptic ulcer.
Methods: Eighty-one male rats were segregated into 9 groups having 9 rats each. Control groups; G-1 (healthycontrol) and G-2 (disease control) were given only distilled water orally. Pantoprazole and Punica granatum peel (PGPE) and seed (PGSE) extracts were given orally once daily to the treatment groups (3-9) for 15 days asfollow: G-3: pantoprazole 60mg/kg/d, G-4: PGPE100mg/kg/d, G-5 PGSE500mg/kg/d, G-6: PGPE50mg/kg/d+ PGSE 250mg/kg/d, G-7: Pantoprazole 30mg/kg/d + PGPE 50mg/kg/d, G-8: Pantoprazole 30mg/kg/d + PGSE 250mg/kg/d, G-9: Pantoprazole 30mg/kg/d + PGPE 50mg/kg/d + PGSE 250mg/kg/d. Groups 2-9 were then given 100mg/kg diclofenac orally on day 17 for induction of toxicity. Gastric parameters (gastric juice volume & pH, ulcer index) were assessed.
Results: All treatment groups demonstrated significant gastroprotective activity against diclofenac. However, group 9 (pantoprazole 30mg/kg/d + PGPE 50mg/kg/d + PGSE 250mg/kg/d) exhibited greater antiulcer activity than pantoprazole as well, shown by improvement in gastric parameters.
Conclusion: The study revealed that Punica granatum peel and seeds, generally considered worthless could be used for prevention of peptic ulcer.

How to cite: Piracha MI, Alam SS, Farooq H, Zulfiqar T, Khan FA, Zahra MEN. Prophylactic Anti-Ulcer Effect of
Punica Granatum (Pomegranate) Peel and Seed Extract in Murine Peptic Ulcer Model. Esculapio - JSIMS
2022;18(04):248-252