Genotypic Comparison of OXA-23 and OXA-48 in Carbapenem Resistant vs. Carbapenem Susceptible Gram Negative Bacteria
DOI:
https://doi.org/10.51273/esc25.251321114Keywords:
OXA-23, OXA-48, Carbapenemase, Oxacillinase, Carbapenem Resistance, Antimicrobial resistance, PCRAbstract
Abstract
Objective: To compare the distribution of OXA-23 and OXA-48 genes in Carbapenem resistant vs.
Carbapenem susceptible gram negative bacteria by PCR.
Material and Methods: It is a comparative cross sectional study conducted in Pathology Department, King
Edward Medical University Lahore. A total of 76 samples were collected, of which 38 were Carbapenem
susceptible and 38 were Carbapenem resistant Gram negative bacteria. Samples were cultured on Blood and
MacConkey agar followed by biochemical testing. Phenotypic Carbapenem susceptibility was evaluated,
and finally, all samples underwent Polymerase Chain Reaction (PCR) for detection of OXA-23 and OXA-48
genes.
Results: In this study OXA-23 gene was isolated in 22 (28.9%) samples and OXA-48 gene was isolated in 5
(6.6%) samples only. OXA-23 gene was found only in Carbapenem resistant samples. However, among
Carbapenem susceptible samples no OXA-23 gene was isolated i.e. p-value<0.001. Similarly, OXA-48 gene
was detected among Carbapenem resistant samples and no OXA-48 gene was found in Carbapenem sensitive
samples i.e. p-value=0.021.
Conclusion: Results of this study show a higher frequency of OXA-23 gene as compared to OXA-48 genes in
Carbapenem resistant gram negative bacteria. More importantly, none of the Carbapenem susceptible
samples had OXA-23 or OXA-48 genes. Meaning that the phenotypic response of bacteria to Carbapenems
was in correspondence with the genotypic results; indicating the reliability of disc diffusion method to
determine Carbapenem resistance in patient samples. This can be very helpful for clinicians for prudent usage
of antibiotics and controlling antimicrobial resistance.
Keywords: OXA-23, OXA-48, Carbapenemase, Antimicrobial resistance, PCR
How to cite: Mahmood S, Bukhari H, Zia Z, Mahmood S. Genotypic Comparison of OXA-23 and OXA-48 in
Carbapenem Resistant vs. Carbapenem Susceptible Gram Negative Bacteria. Esculapio - JSIMS 2025;21(01): 75-79
DOI: https://doi.org/10.51273/esc25.251321114
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