Effect of Moringa Oleifera Leaves on Hepatocytes Glycogen Content After Hepato- toxicity with Bisphenol-A in Albino Rat

Abstract

Objective: To reveal the effects of Moringa Oleifera leaves extract on depleted glycogen stores after hepatotoxicity with Bisphenol-A in albino rats by using PAS staining.
Material and Methods: This experiment was carried out at Post Graduate Medical Institute, 32 adult rats were procured and distributed into 4 groups A, B, C and D. The study duration was 6 weeks. Group A was control and was given corn oil only. Group B, received BPA, 50mg/kg/bw. Group C and D received BPA 50mg/kg along with MoLE 250mg/kg and 500mg/kg. Liver was removed, fixed and slides were prepared by using PAS stain. The number of positively PAS stained (magenta colour) liver cells/40 cells were counted from every group and their mean was compared among groups. The statistical analysis was carried out by applying SPSS 21.
Result: The mean value of PAS positive cells in group B was lowest (14.25±4.27). However, in groups A and D, mean was calculated as 27.6±4.7 and 24.25±5.39, respectively.
Conclusion: Administration of MoLE (250mg/kg & 500mg/kg) restored the depleted glycogen content in cells, which was due to the toxic effect of BPA. The number of cells rises with increasing the dose of MoLE.
Keywords: MoLE: Moringa Oleifera Leave extract, BPA: Bisphenol-A, PAS: Periodic Acid Schiff stain.