Effects of Mesalazine and Coenzyme-Q10 on Colonic Histology in Rat Model of Ulcerative Colitis

Abstract

Objective: To observe the preventive effects of mesalazine and Coenzyme-Q10 on colonic histology in  dextran sulfate sodium induced rat model of Ulcerative Colitis.
Material and Methods: It was an animal experimental study conducted in the Department of Pharmacology, King Edward Medical University and University of Veterinary & Animal Sciences, Lahore. Forty eight 48 healthy male albino rats were divided into 6 groups. Groups A and B were taken as healthy and diseased control groups. Groups B, C, D, E, and F were given 4% Dextran Sulfate Sodium in drinking water for inducing ulcerative colitis. Concomitantly, the rats were treated as per group designation, with full or half doses of mesalazine (50, 100mg/kg/day) and Coenzyme-Q10 (30mg/kg/day). Animals were euthanized after the study and colonic tissue was examined for histopathological changes (extent of inflammation, extent of crypt damage, cryptitis, crypt abscesses, and basal cell plasmacytosis).
Results: There was a marked improvement in the crypt architecture as well as the inflammatory changes in the combined treatment group as compared to the groups given Mesalazine and Coenzyme-Q10 separately.
Conclusion: Combined treatment with Mesalazine and coenzyme-Q10 has a better protective effect on colon in ulcerative colitis than either of them used alone.
Keywords: ulcerative colitis, mesalazine, coenzyme-Q10, histopathological, dextran sulfate sodium