Comparative Cardioprotection Analysis Of Phoenix Dactilyfera. Ajwa Dates (an Ace Inhibitor) with Captopril , In Clozapine Generated Cardiotoxic Rat Model

Abstract

Objective: To compare the cardioprotective effects of Ajwa dates fruit and pit extracts with Captopril in Clozapine induced cardiotoxic rats.
Material and Methods: Place and duration of study at Postgraduate Medical Institute Lahore for 21 days. In an experimental study, forty-eight rats were randomly divided into six groups of eight rats per group. Group 1 was healthy Control group. Myocarditis was induced in all other groups by Clozapine injection. Group 2 was diseased control group while groups 3 – 6 were given Captopril and Ajwa fruit and pit extracts respectively. After 21 days, blood samples were collected from heart and serum was used for further analysis. Tissue samples were collected by homogenizing hearts in phosphate buffer and supernatant used for biochemical testing. Oxidative stress parameters, CK-MB, GSH, GSH-Px, LDH and MDA were studied in all groups.
Results: LDH and CK-MB values of group 6, having captopril 5mg and ajwa fruit and pit both extract, was closest to the control group. Similarly GSH, GSH-Px and MDA values in group 6 were closest to the control group. P-Value came out to be significant for both comparisons hence ensuring the significance of the results.
Conclusion: Captopril and Ajwa dates have strong cardioprotective and antioxidant effects. Patients who are
experiencing adverse effects, may benefit from the combination of Ajwa dates and low-dose captopril.
Keywords: LDH (Lactate dehydrogenase), CK-MB (Creatinine kinase), GSH,GSH-Px (Glutathione peroxidase),
MDA (Malondialdehyde)