Vitamin D Supplementation Reduce Serum Inflammatory Biomarker (TNF-α) Levels in Male Albino Mice on High Fat Diet

Abstract

Objective: The objective of this study was to determine whether vitamin D supplementation affects the levels of inflammatory biomarker (TNF- alpha) in mice (male albino) or not?
Material and Methods: This was a Quasi experimental study. Sample size was 90. Consecutive, nonprobability sampling technique was used. Mice were randomly divided into three groups, each group containing 30 mice. This study was carried out for 6 weeks. Normal diet was administered to mice of group A. High fat diet was administered to group B mice. High fat diet & vitamin D supplementation were given to group C mice. Vitamin D was administered to group C mice through oral gavage (100ng/kg/day) for 6 weeks. Terminal blood sampling technique was used to collect blood sample. ELISA technique was used to determine levels of TNF-α (Tumor necrosis factor alpha). SPSS version 20 was used for analysis of data.
Results: Group B mice had significantly raised serum TNF-α levels as compared to group A mice. Group C mice had significantly reduced levels of tumor necrosis factor-alpha as compared to mice of group B and mice of group A.
Conclusion: Vitamin D supplementation might be beneficial by reducing levels of serum TNF-α levels in mice on high fat diet.
Keywords: Vitamin D, High fat diet, Hyperlipidemia, TNF-α.